Dataset Details
| Title | Lck_Jurkat_AF647_CD3CD28_control |
|---|---|
| Modality | dSTORM |
| Primary Antibody | Lck (D88) XP(R) Rabbit mAb (51ug/ml) |
| Primary Antibody Catalog Number | 2984S |
| Secondary Antibody | AlexaFluor 647 goat anti-rabbit (2mg/ml) IgG |
| Secondary Antibody Catalog Number | |
| Localization Software | SMAP |
| Fluorophore | alexafluor-647 |
| Protein | lck |
| UniProt Protein ID | P06240 |
| Cell Type | Jurkat E6.1 |
| Primary Cell Line | False |
| Cell Line Company | |
| Cell Line Number | |
| DOI | |
| Contact Email for Dataset |
s.shirgill@bham.ac.uk
If you need access to the raw data, please use this email to make your request. Clicking the email link will open your default email client. |
| Drift Corrected | True |
| Blink Corrected | True |
| Effective Pixel Size (nm) | 117.0 |
| Experimental Notes | Jurkat T cells activated on anti-CD3CD28 coated glass surface for 5 mins, then fixed with 4% PFA at room temperature for 10 mins. Cells were permeabilised with 0.1% Triton X-100 in PBS for 3 mins at room temperature followed by blocking in 5% BSA for 30 mins and then stained. Imaging buffer used consisted of 18% glucose, 10 mM Tris (pH 8), 50 mM NaCl, 0.8 mg/mL glucose oxidase, 50 mM cysteamine and 40 ug/mL catalase. |
| Uploaded by | SanShirg |
| Upload date | 25 Nov 2024, 11:32 a.m. |
| Most Similar Dataset | Lck_Jurkat_AF647_SMAP |
| Minimum Dissimilarity Score (thinned) | 0.4900450009433304 |
| Overall Mean Localization Precision (nm) | 12.490 |
Data Files
Self-Similarity Histograms
Self-similarity histograms and statistics for both thinned (left) and unthinned (right) data have been calculated. The dashed red line on both graphs represents the mean.
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Similarity Search Results
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