Dataset Details
| Title | microtubules_cos7_af647_ONI |
|---|---|
| Modality | dSTORM |
| Primary Antibody | beta-tubulin (F-1) mouse monoclonal IgG3 200ug/ml |
| Primary Antibody Catalog Number | sc-166729 |
| Secondary Antibody | AlexaFluor 647 goat anti-rabbit (2mg/ml) IgG |
| Secondary Antibody Catalog Number | |
| Localization Software | SMAP |
| Fluorophore | alexafluor-647 |
| Protein | microtubules |
| UniProt Protein ID | A2AQ07 |
| Cell Type | COS-7 |
| Primary Cell Line | False |
| Cell Line Company | |
| Cell Line Number | |
| DOI | |
| Contact Email for Dataset |
s.shirgill@bham.ac.uk
If you need access to the raw data, please use this email to make your request. Clicking the email link will open your default email client. |
| Drift Corrected | True |
| Blink Corrected | True |
| Effective Pixel Size (nm) | 117.0 |
| Experimental Notes | COS-7 cells extracted and fixed following the protocol in 10.1016/j.ymeth.2019.05.008. Cells were permeabilised with 0.1% Triton X-100 in PBS for 3 mins at room temperature followed by blocking in 5% BSA for 30 mins and then stained. Imaging buffer used consisted of 18% glucose, 10 mM Tris (pH 8), 50 mM NaCl, 0.8 mg/mL glucose oxidase, 50 mM cysteamine and 40 ug/mL catalase. Images were acquired using an ONI Nanoimager. |
| Uploaded by | SanShirg |
| Upload date | 20 Mar 2025, 3:29 p.m. |
| Most Similar Dataset | microtubules_cos7_af647_nocodazole_0.5 |
| Minimum Dissimilarity Score (thinned) | 0.5796429883820596 |
| Overall Mean Localization Precision (nm) | 16.823 |
Data Files
Self-Similarity Histograms
Self-similarity histograms and statistics for both thinned (left) and unthinned (right) data have been calculated. The dashed red line on both graphs represents the mean.
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Similarity Search Results
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- A Dissimilarity Score (thinned) (mean) of "None" indicates that datasets have no grid square densities above the thinning threshold.