Dataset Details
| Title | PSTPIP1-Ab2_Jurkat_aCD3aCD28_5min_20260304 |
|---|---|
| Modality | dSTORM |
| Primary Antibody | Anti-PSTPIP1 antibody SH3 domain (polyclonal) |
| Primary Antibody Catalog Number | NBP3-25077 |
| Secondary Antibody | AlexaFluor 647 goat anti-rabbit (2mg/ml) IgG |
| Secondary Antibody Catalog Number | |
| Localization Software | SMAP |
| Fluorophore | alexafluor-647 |
| Protein | PSTPIP1 |
| UniProt Protein ID | O43586 |
| Subcellular Location (from UniProt) |
|
| Cell Type | Jurkat E6.1 |
| Primary Cell Line | False |
| Cell Line Company | |
| Cell Line Number | |
| DOI | |
| Contact Email for Dataset |
jxl2143@student.bham.ac.uk
If you need access to the raw data, please use this email to make your request. Clicking the email link will open your default email client. |
| Drift Corrected | True |
| Blink Corrected | True |
| Effective Pixel Size (nm) | 117.0 |
| Experimental Notes | Jurkat T cells activated on anti-CD3CD28 coated glass surface for 5 mins, then fixed with 4% PFA at room temperature for 15 mins. Cells were permeabilised with 0.1% Triton X-100 in PBS for 5 min at room temperature, followed by incubation with the primary antibody diluted in 50 mg/mL BSA in 1×PBS for 40 min. The primary was washed off with large volumes of 1×PBS, and the secondary antibody was incubated for 30 min in 50 mg/mL BSA in 1×PBS. Samples were post-fixed with 4% PFA for 3 min at room temperature. Imaging was performed in a dSTORM buffer consisting of 20% glucose, 10 mM Tris (pH 8), 50 mM NaCl, 0.8 mg/mL glucose oxidase, 25 mM cysteamine, and 40 µg/mL bovine catalase. |
| Uploaded by | jl1920 |
| Upload date | 5 Mar 2026, 9:49 p.m. |
| Most Similar Dataset | LAT_Jurkat_aCD90_5min_20260212 |
| Minimum Dissimilarity Score (thinned) | 0.5014029541331242 |
| Overall Mean Localization Precision (nm) | 14.254 |
Data Files
| Title | Modality | Localization Software | Fluorophore | Protein | Subcellular Location | Cell Type | Upload Date |
|---|---|---|---|---|---|---|---|
| Loading data... | |||||||
Self-Similarity Histograms
Self-similarity histograms and statistics for both thinned (left) and unthinned (right) data have been calculated. The dashed red line on both graphs represents the mean.
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Similarity Search Results
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